Biosensor

Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Enzyme Activity (Non-Kinase)

histone H4 lysine acetylation

Histac (K5, K7)

FRET-based biosensors CFP, YFP(venus) Yoshida M
Enzyme Activity (Non-Kinase)

histone H4 lysine acetylation

Histac-K12 (K12)

FRET-based biosensors confocal microscopy COS7 cells CFP, YFP(venus) Yoshida M
Enzyme Activity (Non-Kinase)

histone lysine methylation

K9 reporter

FRET-based biosensors MEF cells CFP, YFP Ting AY
Enzyme Activity (Non-Kinase)

histone lysine methylation

K27 reporter

FRET-based biosensors Ting AY
Enzyme Activity (Non-Kinase)

O-GlcNAc transferase

O-GlcNAc sensor

FRET-based biosensors HeLa cells eCFP, Venus Mahal LK
Enzyme Activity (Non-Kinase)

protein phosphatase (calcineurin)

CaNAR1

FRET-based biosensors HeLa cells eCFP, cpV(L194)(Venus) Zhang J
Enzyme Activity (Non-Kinase)

various proteases

donor FP-substrate-acceptor FP

FRET-based biosensors HeLa cells eCFP, eBFP Luo Y
FRET-based biosensors

PtdIns(4,5)P2

Pippi-PI(4,5)P2

FRET-based biosensors MDCK cells Matsuda M
Ions

pH

GFpH

Single FP-based intrinsic biosensors Miyazaki S
Ions

pH

YFpH

Single FP-based intrinsic biosensors Miyazaki S
Ions

pH

mtAlpHi

Single FP-based intrinsic biosensors Pozzan T
Ions

pH

pHRed

Single FP-based intrinsic biosensors Yellen G
Ions

pH

CFP-YFP tandem

FRET-based biosensors Schaefer M
Ions

PO42-

FLIPPi

FRET-based biosensors confocal flourescence microscopy Kd - 30mM COS-7 cells, CHO cells, E. coli BL21(DE3)Gold cells Synechococcus phosphate-binding protein (PiBP) eCFP, Venus Frommer WB Available
Ions

Zn2+

CLY9-2His

FRET-based biosensors Merkx M
Ions

Zn2+

CALWY

FRET-based biosensors Merkx M
Ions

Zn2+

eCALWYs

FRET-based biosensors Pancreatic beta-cells Rutter GA, Merkx M
Ions

Ca2+

Pericams

Single FP-based extrinsic biosensors Tsien RY, Miyawaki A, Campbell RE
Ions

Zn2+

Cys2His2

FRET-based biosensors Neurons Palmer AE
Ions

Ca2+

GCaMPs

Single FP-based extrinsic biosensors Tsien RY, Imoto K, Kotlikoff MI, Looger LL, Campbell RE, Harvey BK
Ions

Zn2+

ZapCY

FRET-based biosensors HELA cells (Cytosol, Golgi, ER) Palmer AE
Ions

Ca2+

Case12/16

Single FP-based extrinsic biosensors Tsien RY, Chudakov DM, Campbell RE
Ions

Cd2+

Cd-FRET-2

FRET-based biosensors Merkx M
Ions

Ca2+

Camgaroos

Single FP-based extrinsic biosensors Tsien RY, Campbell RE
Ions

Zn2+

ZinCh

FRET-based biosensors Merkx M

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