Filters
Sensor Target Type Specificity Sensor Namesort descending Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Metabolites

cAMP

(T)Epac(VV)

FRET-based biosensors FLIM, confocal fluorescence microscopy cAMP indicators Hek293 cells, N1E-115, human HeLa cells (ccl-2) cAMP binding mTurquise (mECFPD-Epac(CD, and DEP)-cp173Venus-Venus Jalink K
Ions

Cu+

Ace1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Metabolites

glucose

AcGFP1-GBPcys-mCherry

FRET-based biosensors Ye K
Kinase Activity

Protein kinase A

AKAR1-34

FRET-based biosensors HEK293 cells Various Tsien RY, Zhang J, Zhang J
Kinase Activity

Protein kinase B/Akt

Akind

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Kinase Activity

Protein kinase B/Akt

AktAR

FRET-based biosensors NIH 3T3 Cerulean, cpV E172 Zhang J
Kinase Activity

Protein kinase B/Akt

Aktus

FRET-based biosensors CHO cells CFP, YFP Umezawa Y
Ions

Cu+

Amt1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive, Kd for Cu+ binding: 2.5x10^-18 M E.coli, yeast cells, CHO-K1 cells Copper binding domain of Amt1 CFP, YFP He C
Metabolites

NADP+

Apollo-NADP++

Steady-state fluorescence anisotropy due to FRET Cerulean Rocheleau JV
Kinase Activity

Protein kinase A

ART

FRET-based biosensors COS-7 cells BFP, RFP Hagiwara, M
Metabolites

ATP

ATeams

FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Ions

Ca2+

B-GECO

Single FP-based extrinsic biosensors Tsien RY, Campbell RE
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Kinase Activity

Protein kinase B/Akt

BKAR

FRET-based biosensors NIH 3T3 mCFP, mYFP Newton AC
Ions

Zn2+

CALWY

FRET-based biosensors Merkx M
Ions

Ca2+

Cameleons

FRET-based biosensors Tsien RY, Miyawaki A, Tsien RY, Persechini A, Ikura M
Ions

Ca2+

Camgaroos

Single FP-based extrinsic biosensors Tsien RY, Campbell RE
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y
Enzyme Activity (Non-Kinase)

protein phosphatase (calcineurin)

CaNAR1

FRET-based biosensors HeLa cells eCFP, cpV(L194)(Venus) Zhang J

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