Sensor Target Type |
Specificity![]() |
Sensor Name | Sensor Type | Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs | Addgene Link |
---|---|---|---|---|---|---|---|---|---|---|
Metabolites | FRET-Based sensor | confocal microscopy | Kd 1: 8 uM/ Kd 2: 800 uM | HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons | LldR | mTFP, Venus | Felipe Barros | Available | ||
Metabolites | FRET-Based sensor | confocal microscopy | Kd: 107 uM | HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons | PdhR | mTFP, Venus | Felipe Barros | Available | ||
Metabolites |
1,2-diacylglycerol |
Cys1-GFP |
Translocation-based biosensors | Meyer T | ||||||
Metabolites |
1,2-diacylglycerol |
DAGR (FRET) |
Translocation-based biosensors | Newton AC | ||||||
Kinase Activity |
Abl |
Crk-based reporter |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific (tyrosine) | NIH 3T3, MEF cells | complete chicken c-CrkII gene | eCFP, eYFP (citrine) | Tsien RY | |
Kinase Activity |
Abl |
EGFR indicator |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific, sensitive to concentration of EGF | B82, NIH 3T3, HeLa cells | SH2 domain (residues 374–465) from mouse p52 Shc | eCFP, eYFP (citrine) | Tsien RY | |
Kinase Activity |
Abl |
Src indicator |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific (tyrosine) | NIH 3T3, HeLa Cells | Rous sarcoma virus Src gene (residues148–245) | eCFP, eYFP (citrine) | Tsien RY | |
Kinase Activity |
Abl |
Picchu |
FRET-based biosensors | fluorescence microscopy | Kinase Specific (tyrosine) | HeLa cells, Cos7/E3 | Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) | eYFP, CFP | Matsuda M | |
Kinase Activity |
Abl |
Picchu-Z/EGFR-Z |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific (tyrosine) | Cos1 and HT1080 and NIH3T3 and A431 | Src-homology (SH) 2 and SH3 from Crk II | YFP, CFP | Matsuda M | |
Protein Activation and Conformation |
activation of plasma membrane Ca2+ pump |
BFP-PMCA-GFP |
FRET-based biosensors | spectroscopy | concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM | Saccharomyces cerevisiae strain DBY 2062, yeast cells | Ca2+-calmodulin binding | BFP, GFP | Adamo HP | |
Protein Activation and Conformation |
Akt conformation |
ReAktion |
FRET-based biosensors | fluorescence microscopy | NIH3T3 fibroblasts | human Akt-1/protein kinase B-a | eCFP, Citrine | Zhang J | ||
Protein Activation and Conformation |
Akt conformation |
GFP–Akt–YFP |
FRET-based biosensors | FLIM | NIH 3T3 | protein kinase B (PKB)/Akt | eGFP , eYFP | Larijani B | ||
Metabolites |
amino acids |
FLIPs for amino acids |
FRET-based biosensors | fluorescence microscopy, spectroscopy | cross-binding of other aa | Yeast cells | circularly permutated PBPs | eCFP, Venus | Ito T | |
Protein Activation and Conformation |
Annexin A4 self-association |
CYNEX4 |
FRET-based biosensors | confocal flourescence microscopy, FRAP | HeLa cells, N1E-115, MDCK2 neuroblastoma cells | Annexin A4 (Ca2+ regulated phospholated) | eCFP, eYFP | Schultz C | ||
Metabolites |
arabinose |
FLIParaF.Ec-250n |
FRET-based biosensors | spectroscopy | Kd-230nM ± 18 nM | E. coli BL21-Gold(DE3) | E.coli K12 araF | eCFP, Venus | Frommer WB | Available |
Metabolites |
arginine |
FRET arginine reporter |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM | E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots | bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N | eCFP, Citrine (shortened) | Ludewig U | |
Kinase Activity |
ATM-Protein Kinase |
ATOMIC |
FRET-based biosensors | confocal flourescence microscopy | irreversible | HeLa cells, NIH 3T3, AT4Bi | Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site | CFP, YFP | Hunter T | |
Metabolites |
ATP |
ATeams |
FRET-based biosensors | spectroscopy, fluorescence microscopy | ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. | HeLa cells, NIH 3T3 | ? subunit from Bacillus subtilis FoF1-ATP synthase | CFP (mseCFP), YFP (cp173-mVenus) | Noji H | |
Metabolites |
ATP:ADP ratio |
Perceval |
Single FP-based extrinsic biosensors | Yellen G | ||||||
Kinase Activity |
Aurora B kinase |
aurora B sensor |
FRET-based biosensors | confocal flourescence microscopy | Xenopus S3, HeLa Cells, DU145, DLD21 | reversible binding to an FHA2 phospho-threonine binding domain | CFP, YFP | Kapoor TM | ||
Metabolites |
BAI2 (a furanosyl borate diester) |
CLPY |
FRET-based biosensors | fluorescence microscopy | 98 kDa (CLPY), BAI2 Kd 270 nM | BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs | LuxP-BAI2 binding | CFP, YFP | Sayre R | |
Metabolites |
BAI2 (a furanosyl borate diester) |
LuxP-FRET |
FRET-based biosensors | spectroscopy, fluorescence microscopy | BAI2 Kd 270 nM | Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) | LuxP-BAI2 binding | CFP, YFP | Sayre R | |
Ions |
Ca2+ |
Pericams |
Single FP-based extrinsic biosensors | Tsien RY, Miyawaki A, Campbell RE | ||||||
Ions |
Ca2+ |
GCaMPs |
Single FP-based extrinsic biosensors | Tsien RY, Imoto K, Kotlikoff MI, Looger LL, Campbell RE, Harvey BK | ||||||
Ions |
Ca2+ |
Case12/16 |
Single FP-based extrinsic biosensors | Tsien RY, Chudakov DM, Campbell RE |