Filters
Sensor Target Type Specificitysort descending Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Metabolites

1,2-diacylglycerol

Cys1-GFP

 Translocation-based biosensors Meyer T
Metabolites

1,2-diacylglycerol

DAGR (FRET)

 Translocation-based biosensors Newton AC
Kinase Activity

Abl

Crk-based reporter

 FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, MEF cells complete chicken c-CrkII gene eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

EGFR indicator

 FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

Src indicator

 FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

Picchu

 FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Kinase Activity

Abl

Picchu-Z/EGFR-Z

 FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

 FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Protein Activation and Conformation

Akt conformation

ReAktion

 FRET-based biosensors fluorescence microscopy NIH3T3 fibroblasts human Akt-1/protein kinase B-a eCFP, Citrine Zhang J
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

 FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Metabolites

amino acids

FLIPs for amino acids

 FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

 FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Metabolites

arabinose

FLIParaF.Ec-250n

 FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Metabolites

arginine

FRET arginine reporter

 FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U
Kinase Activity

ATM-Protein Kinase

ATOMIC

 FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Metabolites

ATP

ATeams

 FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H
Metabolites

ATP:ADP ratio

Perceval

 Single FP-based extrinsic biosensors Yellen G
Kinase Activity

Aurora B kinase

aurora B sensor

 FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

 FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

 FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Ions

Ca2+

Pericams

 Single FP-based extrinsic biosensors Tsien RY, Miyawaki A, Campbell RE
Ions

Ca2+

GCaMPs

 Single FP-based extrinsic biosensors Tsien RY, Imoto K, Kotlikoff MI, Looger LL, Campbell RE, Harvey BK
Ions

Ca2+

Case12/16

 Single FP-based extrinsic biosensors Tsien RY, Chudakov DM, Campbell RE

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