| Sensor Target Type | Specificity | Sensor Name |
Sensor Type |
Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs | Addgene Link |
|---|---|---|---|---|---|---|---|---|---|---|
| Metabolites |
PtdIns3P |
GFP–p40phox iPX |
Stephens LR | |||||||
| Enzyme Activity (Non-Kinase) |
histone H4 lysine acetylation |
Histac (K5, K7) |
FRET-based biosensors | CFP, YFP(venus) | Yoshida M | |||||
| Metabolites |
histidine |
FLIP-HisJ/cpHisJ194 |
FRET-based biosensors | Ito T | ||||||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR reporter |
FRET-based biosensors | HeLa cells, NIH 3T3 | CFP, YFP | Tsien RY | ||||
| Protein Activation and Conformation |
Akt conformation |
ReAktion |
FRET-based biosensors | fluorescence microscopy | NIH3T3 fibroblasts | human Akt-1/protein kinase B-a | eCFP, Citrine | Zhang J | ||
| Metabolites |
inositol 1,4,5-trisphosphate |
LIBRA |
FRET-based biosensors | Mikoshiba K | ||||||
| Redox and Voltage |
voltage |
VSFP-cpEGFP |
FRET-based biosensors | Sakai R, Isacoff EY | ||||||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
ECaus |
FRET-based biosensors | fluorescence microscopy, spectroscopy | Kinase Specific | CHO-EGFR cells | Substrate, SH2 domain | CFP, YFP | Umezawa Y | |
| Metabolites |
arabinose |
FLIParaF.Ec-250n |
FRET-based biosensors | spectroscopy | Kd-230nM ± 18 nM | E. coli BL21-Gold(DE3) | E.coli K12 araF | eCFP, Venus | Frommer WB | Available |
| Protein Activation and Conformation |
Akt conformation |
GFP–Akt–YFP |
FRET-based biosensors | FLIM | NIH 3T3 | protein kinase B (PKB)/Akt | eGFP , eYFP | Larijani B | ||
| Protein Activation and Conformation |
Annexin A4 self-association |
CYNEX4 |
FRET-based biosensors | confocal flourescence microscopy, FRAP | HeLa cells, N1E-115, MDCK2 neuroblastoma cells | Annexin A4 (Ca2+ regulated phospholated) | eCFP, eYFP | Schultz C | ||
| Metabolites |
inositol 1,4,5-trisphosphate |
FIRE1-3 |
FRET-based biosensors | Mignery GA | ||||||
| Redox and Voltage |
voltage |
VSFPs |
FRET-based biosensors | Knöpfel T, Knöpfel T, Knöpfel T, Knöpfel T | ||||||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
Bescell/ BBD-Ecat |
FRET-based biosensors | fluorescence microscopy, spectroscopy | Kinase specific, selective to BDNF | MCF-7 cells | BDNF binding to BBD-Ecat domain | CFP, YFP | ||
| Metabolites |
arginine |
FRET arginine reporter |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM | E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots | bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N | eCFP, Citrine (shortened) | Ludewig U | |
| Protein Activation and Conformation |
CaMKII conformation |
CamuiαamCYCaMIIα |
FRET-based biosensors | two-photon fluorescence microscopy | HeLa cells | YFP(Venus) , CFP (S175G) | Schultz C, Hayashi Y | |||
| Metabolites |
inositol 1,4,5-trisphosphate |
IRIS-1 |
FRET-based biosensors | Tojyo Y | ||||||
| Redox and Voltage |
voltage |
Mermaid |
FRET-based biosensors | Neuro2A cells | CFP, YFP | Miyawaki A | ||||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR-ECFP/PTV-EYFP |
FRET-based biosensors | FLIM, fluorescence microscopy | PTB domain (KD, 140 nM to 5.3 µM) | MCF-7 cells | Phosphotyrosine (PTB) domain of human Shc | eCFP, eYFP (citrine) | Bastiaens PI | |
| Metabolites |
amino acids |
FLIPs for amino acids |
FRET-based biosensors | fluorescence microscopy, spectroscopy | cross-binding of other aa | Yeast cells | circularly permutated PBPs | eCFP, Venus | Ito T | |
| Protein Activation and Conformation |
ERK conformation |
Miu2 |
FRET-based biosensors | HeLa cells, COS-7 cells, 293F | CFP, YFP, RFP(Dropna) | Matsuda M | ||||
| Metabolites |
leucine |
FLIP-LivJ/cpLivJ261 |
FRET-based biosensors | Ito T | ||||||
| Redox and Voltage |
voltage |
FlaSh-CFP/YFP |
FRET-based biosensors | salamander retinal cones, on-bipolar cells, horizontal cells, wide-field amacrine cells | eGFP, GFPuv, and wtGFP, eCFP and eYFP | Isacoff EY | ||||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR-ECFP/PTV-EYFP |
FRET-based biosensors | FLIM, fluorescence microscopy | PTB domain (KD, 140 nM to 5.3 µM) | COS-7 cells | PTB domain | eCFP, eYFP | Bastiaens PI | |
| Metabolites |
ATP |
ATeams |
FRET-based biosensors | spectroscopy, fluorescence microscopy | ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. | HeLa cells, NIH 3T3 | ? subunit from Bacillus subtilis FoF1-ATP synthase | CFP (mseCFP), YFP (cp173-mVenus) | Noji H |