Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Kinase Activity

Protein kinase B/Akt

Aktus

FRET-based biosensors CHO cells CFP, YFP Umezawa Y
Metabolites

cGMP

cGi sensors

FRET-based biosensors spectroscopy, fluorescence microscopy cGMP concentration 1-2 µM HEK293 cells cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) eCFP, eYFP Koesling D
Protein Activation and Conformation

Rho family GTPase activation

RhoA biosensor commentary

FRET-based biosensors Hahn KM, Hahn KM
Ions

Cd2+

Cd-FRET-2

FRET-based biosensors Merkx M
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors MDCK cells Matsuda M
Kinase Activity

Protein kinase B/Akt

Akind

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Metabolites

cGMP

Cygnus

FRET-based biosensors FLIM, confocal fluorescence microscopy, spectroscopy pH-sensitive PC12 cells, HEK 293T cells, HeLa cells cGMP binding from PDE5 mTagBFP, sREACh(dark) Oka K
Protein Activation and Conformation

Wiskott -Aldrich syndrome protein (WASP) conformation

Cdc42-GEF sensors

FRET-based biosensors NIH 3T3 cells eCFP, eYFP Rosen MK
Ions

Zn2+

ZinCh

FRET-based biosensors Merkx M
Metabolites

PtdIns(3,4,5)P3

Fllip

FRET-based biosensors CHO cells Umezawa Y
Kinase Activity

Protein kinase B/Akt

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 GFP, RFP Larijani B
Metabolites

citrate

CIT sensors

FRET-based biosensors fluorescence microscopy Kd range - 8 µM - 1765 µM Kd: CFP-CitA6-130-Venus - 8 µM COS cells, arabidopsis, yeast, e.coli citrate binding of the Klebsiella pneumoniae histidine sensor kinase CitA venus, CFP Zamboni N
Metabolites

PtdIns(3,4,5)P3

Pippi-PI(3,4,5)P3

FRET-based biosensors COS-7 cells Matsuda M
Kinase Activity

Protein kinase B/Akt

ReAktion

FRET-based biosensors NIH 3T3 CFP, YFP Zhang J
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Kinase Activity

Protein kinase B/Akt

AktAR

FRET-based biosensors NIH 3T3 Cerulean, cpV E172 Zhang J
Metabolites

ribose

FLIPrib sensors

FRET-based biosensors Lalonde S
Metabolites

diacylglycerol

DAGR

FRET-based biosensors confocal flourescence microscopy MDCK cells Cysteine-rich domain (CRD) CFP, YFP Umezawa Y
Redox and Voltage

redox

CY-RL5 and CY-RL7

FRET-based biosensors CFP and eYFP, mCyPet and mYPet Gaskins HR, Gaskins HR
Kinase Activity

Protein kinase C

CKAR; KCP-1

FRET-based biosensors Schultz C, Newton AC
Metabolites

sucrose

FLIPsuc sensors

FRET-based biosensors Frommer WB
Ions

Ca2+

Cameleons

FRET-based biosensors Tsien RY, Miyawaki A, Tsien RY, Persechini A, Ikura M
Ions

Ca2+

FIP-CBSMTN-XL

FRET-based biosensors Miyawaki A, Persechini A,, Ikura M
Metabolites

diacylglycerol

CKAR

FRET-based biosensors fluorescence microscopy, spectroscopy Sensitive to calyculcin A and PKC HeLa cells, MDCK cells FHA2 domain of Rad53p mCFP, mYFP Newton AC
Redox and Voltage

redox

HSP-FRET

FRET-based biosensors CFP, YFP Schumacker PT

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