Filters
Sensor Target Type Specificity Sensor Name Sensor Typesort descending Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Metabolites

glutamate

GluSnFR

FRET-based biosensors Tsien RY
Kinase Activity

Abl

Picchu-Z/EGFR-Z

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Metabolites

PtdIns(3,4)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Ions

Cu+

Mac1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive Yeast cells, E.coli Copper (I) binding domain CFP, YFP He C
Metabolites

glutamate

FLIP-cpGltI210

FRET-based biosensors Ito T
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors MDCK cells Matsuda M
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Ions

Cu+

Amt1-FRET

FRET-based biosensors fluorescence microscopy, spectroscopy Cu+ sensitive, Kd for Cu+ binding: 2.5x10^-18 M E.coli, yeast cells, CHO-K1 cells Copper binding domain of Amt1 CFP, YFP He C
Metabolites

glutamate

FRET glutamine reporter

FRET-based biosensors Ludewig U
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Metabolites

PtdIns(3,4)P2

Pippi-PI(3,4)P2

FRET-based biosensors COS-7 cells Matsuda M
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Metabolites

glutamate

FLIP-cpGlnH183

FRET-based biosensors Ito T
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Metabolites FRET-Based sensor confocal microscopy Kd 1: 8 uM/ Kd 2: 800 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons LldR mTFP, Venus Felipe Barros Available
Metabolites FRET-Based sensor confocal microscopy Kd: 107 uM HeLa cells, Astrocytes, HEK293 cells, hippocampal neurons PdhR mTFP, Venus Felipe Barros Available
Redox and Voltage

voltage

SPARC

Single FP-based extrinsic biosensors Pieribone VA, Sakai R, Isacoff EY
Metabolites

ATP:ADP ratio

Perceval

Single FP-based extrinsic biosensors Yellen G
Regulated Proteolysis

Cell cycle progression

Fucci

Single FP-based extrinsic biosensors COS-7 cells, HeLa Cells RFP, GFP Miyawaki A
Protein Activation and Conformation

Insulin receptor activation

Sinphos

Single FP-based extrinsic biosensors CHO-IR cells, CHO-K1 cells CFP(cpFP), GFP(cpFP) Umezawa Y
Metabolites

cGMP

FlincG

Single FP-based extrinsic biosensors Dostmann WR

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