Biosensor | DPB/DGE Codex

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Sensor Target Type	Specificity	Sensor Name	Sensor Type	Analysis Technique	IV Properties	Organism	Binding Protein	Fluorophore	PIs	Sequence
Ions	Ca²⁺	B-GECO	Single FP-based extrinsic biosensors						Tsien RY, Campbell RE
Ions	Ca²⁺	jRCaMP1a, b	Single FP-based extrinsic biosensors			Cultured neurons		mRuby	Kim DS
Ions	Ca²⁺	G-GECO	Single FP-based extrinsic biosensors						Tsien RY, Campbell RE
Ions	Ca²⁺	jRGECO1a	Single FP-based extrinsic biosensors			Cultured neurons		mApple	Kim DS
Ions	Ca²⁺	R-GECO	Single FP-based extrinsic biosensors						Tsien RY, Campbell RE
Ions	pH	pHuji	Single FP-based intrinsic biosensors					mApple	Campbell RE
Ions	Ca²⁺	Cameleons	FRET-based biosensors						Tsien RY, Miyawaki A, Tsien RY, Persechini A, Ikura M
Ions	Ca²⁺	FIP-CB_SMTN-XL	FRET-based biosensors						Miyawaki A, Persechini A,, Ikura M
Ions	Ca²⁺	TN-XXL	FRET-based biosensors						Griesbeck O, Miyawaki A, Persechini A, Ikura M
Ions	Cl^-	Clomeleon	FRET-based biosensors	confocal flourescence microscopy	PKD1 (K467A) Kd: .196 ÂµM, WT PKD1 - .012, ÂµM, PKD1 (K467R) - .009 ÂµM	E.coli, HEK-293 cells	PDK1 PH domain	green fluorescent protein (GFP-) tagged versions of wild-type PDK1	van Aalten DM
Ions	Cl^-	Cl-sensor	FRET-based biosensors	fluorescence microscopy, spectroscopy	pH-specific/sensitive	CHO-K1 cells, HEK cells, hippocampal neurons, photoreceptors from rat retina	peptide linker	mutated eYFP, CFP	Bregestovski P	test_sequence_ bregestovski.txt
Ions	Cu⁺	Ace1-FRET	FRET-based biosensors	fluorescence microscopy, spectroscopy	Cu+ sensitive	Yeast cells, E.coli	Copper (I) binding domain	CFP, YFP	He C
Ions	Cu⁺	Mac1-FRET	FRET-based biosensors	fluorescence microscopy, spectroscopy	Cu+ sensitive	Yeast cells, E.coli	Copper (I) binding domain	CFP, YFP	He C
Ions	Cu⁺	Amt1-FRET	FRET-based biosensors	fluorescence microscopy, spectroscopy	Cu+ sensitive, Kd for Cu+ binding: 2.5x10^-18 M	E.coli, yeast cells, CHO-K1 cells	Copper binding domain of Amt1	CFP, YFP	He C
Ions	halide ions	YFP-H148Q	Single FP-based intrinsic biosensors						Verkman AS
Ions	halide ions	YFP-H148QI152L	Single FP-based intrinsic biosensors						Verkham AS
Ions	Hg²⁺	eGFP205C	Single FP-based intrinsic biosensors						Sagermann M
Ions	Hg²⁺	IFP/BV sensor	Single FP-based intrinsic biosensors						Tang Y
Ions	pH	ECFP	Single FP-based intrinsic biosensors						Tsien RY, Verkman AS
Ions	pH	EYFP	Single FP-based intrinsic biosensors						Tsien RY, Verkman AS
Ions	pH	E²GFP	Single FP-based intrinsic biosensors						Beltram F
Ions	pH	deGFP1-4	Single FP-based intrinsic biosensors						Remington SJ
Ions	pH	pHluorins	Single FP-based intrinsic biosensors						Rothman JE
Kinase Activity	Extracellular signal-regulated kinase (ERK)	Miu2	FRET-based biosensors			HeLa cells		CFP, YFP	Matsuda M
Kinase Activity	Glucokinase	mCerulean-GCK-mVenus	FRET-based biosensors	confocal flourescence microscopy		BTC3 cells		YFP	Rizzo MA

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