Sensor Target Type |
Specificity | Sensor Name | Sensor Type | Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs |
|---|---|---|---|---|---|---|---|---|---|
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR reporter |
FRET-based biosensors | HeLa cells, NIH 3T3 | CFP, YFP | Tsien RY | |||
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
ECaus |
FRET-based biosensors | fluorescence microscopy, spectroscopy | Kinase Specific | CHO-EGFR cells | Substrate, SH2 domain | CFP, YFP | Umezawa Y |
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
Bescell/ BBD-Ecat |
FRET-based biosensors | fluorescence microscopy, spectroscopy | Kinase specific, selective to BDNF | MCF-7 cells | BDNF binding to BBD-Ecat domain | CFP, YFP | |
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR-ECFP/PTV-EYFP |
FRET-based biosensors | FLIM, fluorescence microscopy | PTB domain (KD, 140 nM to 5.3 µM) | MCF-7 cells | Phosphotyrosine (PTB) domain of human Shc | eCFP, eYFP (citrine) | Bastiaens PI |
| Kinase Activity |
epidermal growth factor receptor (EGFR) |
EGFR-ECFP/PTV-EYFP |
FRET-based biosensors | FLIM, fluorescence microscopy | PTB domain (KD, 140 nM to 5.3 µM) | COS-7 cells | PTB domain | eCFP, eYFP | Bastiaens PI |
| Kinase Activity |
Extracellular signal-regulated kinase (ERK) |
Erkus |
FRET-based biosensors | confocal flourescence microscopy | Sensitive to ERK activity | MCF-7 cells | FHA2 domain and ERK docking domain | eCFP, eYFP | Umezawa Y |
| Kinase Activity |
Extracellular signal-regulated kinase (ERK) |
EAS |
FRET-based biosensors | spectroscopy | Sensitive to ERK activity | BL21(DE3) cells, NIH 3T3 | Serine-Proline (SP) binding, either D-domain or DEF domain | eCFP, eYFP | Alberola-Ila J |
| Kinase Activity |
Extracellular signal-regulated kinase (ERK) |
EKAR |
FRET-based biosensors | FLIM, fluorescence microscopy | Sensitive to p38 and JNK and ERK activities | HEK293 cells, pyramidal neurons, COS-7 cells | Phospho-binding domain and ERK docking domain | eGFP , mRFP1 or CFP , YFP | Svoboda K |
| Mechanical Force |
Mechanical strain |
VinTS |
FRET-based biosensors | confocal microscopy | BAECs, MEF cells, HEK293 cells | mTFP1, venus(A206K) | Schwartz MA | ||
| Mechanical Force |
Mechanical strain |
stFRET |
FRET-based biosensors | Dictyostelium , HS1, AX2 | GFP, eGFP | Uyeda TQ | |||
| Mechanical Force |
Mechanical strain |
PriSSM (PRIM-based) |
FRET-based biosensors | BAECs, HEK cells | Cerulean, Venus | Sachs F | |||
| Mechanical Force |
Mechanical strain |
sstFRET |
FRET-based biosensors | confocal microscopy | HEK-293, 3T3, C.Elegans | Cerulean, Venus | Sachs F | ||
| Metabolites |
Phosphatidylserine |
GFP-Lact-C2 |
Translocation-based biosensors | Grinstein S | |||||
| Metabolites |
PtdIns(4,5)P2 |
PHOSBP |
Translocation-based biosensors | confocal microscopy | COS-7 cells | GFP, mRFP | Balla T, Munro S | ||
| Metabolites |
cAMP |
FP-PKARII/FP-PKAcat |
FRET-based biosensors | eBFP and GFP or CFP and YFP | Pozzan T, Pozzan T | ||||
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-ARNO |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
PtdIns(4,5)P2 |
GFP-PHFAPP |
Translocation-based biosensors | confocal microscopy | HeLa cells, yeast cells | eGFP | Lemmon MA | ||
| Metabolites |
cAMP |
CFP–Epac–YFP |
FRET-based biosensors | fluorescence microscopy | highly sensitive cAMP indicator, Kd of 2.3 µM | human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells | cAMP binding | CFP, YFP | Jalink K |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHGRP1 |
Translocation-based biosensors | confocal microscopy | PC12 cells | GFP | Cullen PJ, Czech MP | ||
| Metabolites |
PtdIns(4,5)P2 |
CAY |
FRET-based biosensors | Swiss 3T3 fibroblasts | Allen PG | ||||
| Metabolites |
cAMP |
ICUEs |
FRET-based biosensors | eCFP, Citrine | Zhang J, Zhang J, Lefkowitz RJ | ||||
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-Cytohesin-1 |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
cAMP |
Epac-camps |
FRET-based biosensors | spectroscopy, fluorescence microscopy | sensitive cAMP indicators | Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells | cAMP binding | eCFP, eYFP | Lohse MJ |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHPDK1 |
Translocation-based biosensors | Alessi DR, van Aalten DM | |||||
| Metabolites |
PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics |
GFP-PHPLC- |
Translocation-based biosensors | Nash MS |