Filters
Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR reporter

FRET-based biosensors HeLa cells, NIH 3T3 CFP, YFP Tsien RY
Kinase Activity

epidermal growth factor receptor (EGFR)

ECaus

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific CHO-EGFR cells Substrate, SH2 domain CFP, YFP Umezawa Y
Kinase Activity

epidermal growth factor receptor (EGFR)

Bescell/ BBD-Ecat

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase specific, selective to BDNF MCF-7 cells BDNF binding to BBD-Ecat domain CFP, YFP
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) MCF-7 cells Phosphotyrosine (PTB) domain of human Shc eCFP, eYFP (citrine) Bastiaens PI
Kinase Activity

epidermal growth factor receptor (EGFR)

EGFR-ECFP/PTV-EYFP

FRET-based biosensors FLIM, fluorescence microscopy PTB domain (KD, 140 nM to 5.3 µM) COS-7 cells PTB domain eCFP, eYFP Bastiaens PI
Kinase Activity

Extracellular signal-regulated kinase (ERK)

Erkus

FRET-based biosensors confocal flourescence microscopy Sensitive to ERK activity MCF-7 cells FHA2 domain and ERK docking domain eCFP, eYFP Umezawa Y
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EAS

FRET-based biosensors spectroscopy Sensitive to ERK activity BL21(DE3) cells, NIH 3T3 Serine-Proline (SP) binding, either D-domain or DEF domain eCFP, eYFP Alberola-Ila J
Kinase Activity

Extracellular signal-regulated kinase (ERK)

EKAR

FRET-based biosensors FLIM, fluorescence microscopy Sensitive to p38 and JNK and ERK activities HEK293 cells, pyramidal neurons, COS-7 cells Phospho-binding domain and ERK docking domain eGFP , mRFP1 or CFP , YFP Svoboda K
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Metabolites

PtdIns(4,5)P2

PHOSBP

Translocation-based biosensors confocal microscopy COS-7 cells GFP, mRFP Balla T, Munro S
Metabolites

cAMP

FP-PKARII/FP-PKAcat

FRET-based biosensors eBFP and GFP or CFP and YFP Pozzan T, Pozzan T
Metabolites

Phosphatidylserine

GFP-Lact-C2

Translocation-based biosensors Grinstein S
Metabolites

PtdIns(4,5)P2

GFP-PHFAPP

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Metabolites

cAMP

CFP–Epac–YFP

FRET-based biosensors fluorescence microscopy highly sensitive cAMP indicator, Kd of 2.3 µM human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells cAMP binding CFP, YFP Jalink K
Metabolites

PtdIns(3,4,5)P3

GFP-ARNO

Translocation-based biosensors Czech MP
Metabolites

PtdIns(4,5)P2

CAY

FRET-based biosensors Swiss 3T3 fibroblasts Allen PG
Metabolites

cAMP

ICUEs

FRET-based biosensors eCFP, Citrine Zhang J, Zhang J, Lefkowitz RJ
Metabolites

PtdIns(3,4,5)P3

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ, Czech MP
Metabolites

cAMP

Epac-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ
Metabolites

PtdIns(3,4,5)P3

GFP-Cytohesin-1

Translocation-based biosensors Czech MP
Metabolites

PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics

GFP-PHPLC-

Translocation-based biosensors Nash MS
Metabolites

cAMP

PKA-camps

FRET-based biosensors spectroscopy, fluorescence microscopy sensitive cAMP indicators Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells cAMP binding eCFP, eYFP Lohse MJ

Pages