Sensor Target Type |
Specificity | Sensor Name | Sensor Type | Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs | Addgene Link |
|---|---|---|---|---|---|---|---|---|---|---|
| Kinase Activity |
Abl |
EGFR indicator |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific, sensitive to concentration of EGF | B82, NIH 3T3, HeLa cells | SH2 domain (residues 374–465) from mouse p52 Shc | eCFP, eYFP (citrine) | Tsien RY | |
| Kinase Activity |
Src |
Srcus |
FRET-based biosensors | HeLa cells, MEF cells | CFP, YFP | Chien S | ||||
| Kinase Activity |
Abl |
Src indicator |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific (tyrosine) | NIH 3T3, HeLa Cells | Rous sarcoma virus Src gene (residues148–245) | eCFP, eYFP (citrine) | Tsien RY | |
| Kinase Activity |
Abl |
Picchu |
FRET-based biosensors | fluorescence microscopy | Kinase Specific (tyrosine) | HeLa cells, Cos7/E3 | Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) | eYFP, CFP | Matsuda M | |
| Kinase Activity |
Abl |
Picchu-Z/EGFR-Z |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | Kinase Specific (tyrosine) | Cos1 and HT1080 and NIH3T3 and A431 | Src-homology (SH) 2 and SH3 from Crk II | YFP, CFP | Matsuda M | |
| Kinase Activity |
ATM-Protein Kinase |
ATOMIC |
FRET-based biosensors | confocal flourescence microscopy | irreversible | HeLa cells, NIH 3T3, AT4Bi | Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site | CFP, YFP | Hunter T | |
| Kinase Activity |
Aurora B kinase |
aurora B sensor |
FRET-based biosensors | confocal flourescence microscopy | Xenopus S3, HeLa Cells, DU145, DLD21 | reversible binding to an FHA2 phospho-threonine binding domain | CFP, YFP | Kapoor TM | ||
| Kinase Activity |
CyclinB-Cdk1 |
CyclinB-Cdk1 sensor |
FRET-based biosensors | fluorescence microscopy, spectroscopy | Kinase Specific | HeLa cells, Xenopus, MEF, hTert-RPE1 cells | Polo box domain (PBD) of PIk1 | CFP(mCerulean) , YFP(YPet) | Pines J | |
| Mechanical Force |
Mechanical strain |
VinTS |
FRET-based biosensors | confocal microscopy | BAECs, MEF cells, HEK293 cells | mTFP1, venus(A206K) | Schwartz MA | |||
| Mechanical Force |
Mechanical strain |
stFRET |
FRET-based biosensors | Dictyostelium , HS1, AX2 | GFP, eGFP | Uyeda TQ | ||||
| Mechanical Force |
Mechanical strain |
PriSSM (PRIM-based) |
FRET-based biosensors | BAECs, HEK cells | Cerulean, Venus | Sachs F | ||||
| Mechanical Force |
Mechanical strain |
sstFRET |
FRET-based biosensors | confocal microscopy | HEK-293, 3T3, C.Elegans | Cerulean, Venus | Sachs F | |||
| Metabolites |
1,2-diacylglycerol |
DAGR (FRET) |
Translocation-based biosensors | Newton AC | ||||||
| Metabolites |
histidine |
FLIP-HisJ/cpHisJ194 |
FRET-based biosensors | Ito T | ||||||
| Metabolites |
PtdIns(3,5)P2 |
GFP-PHPDK1 |
Translocation-based biosensors | Alessi DR | ||||||
| Metabolites |
arabinose |
FLIParaF.Ec-250n |
FRET-based biosensors | spectroscopy | Kd-230nM ± 18 nM | E. coli BL21-Gold(DE3) | E.coli K12 araF | eCFP, Venus | Frommer WB | Available |
| Metabolites |
inositol 1,4,5-trisphosphate |
LIBRA |
FRET-based biosensors | Mikoshiba K | ||||||
| Metabolites |
PtdIns(3,5)P2 |
YFP-PHTAPP1 |
Translocation-based biosensors | Alessi DR | ||||||
| Metabolites |
arginine |
FRET arginine reporter |
FRET-based biosensors | confocal flourescence microscopy, spectroscopy | pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM | E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots | bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N | eCFP, Citrine (shortened) | Ludewig U | |
| Metabolites |
inositol 1,4,5-trisphosphate |
FIRE1-3 |
FRET-based biosensors | Mignery GA | ||||||
| Metabolites |
PtdIns(3,5)P2 |
GFP-Svp1p |
Translocation-based biosensors | Lemmon MA | ||||||
| Metabolites |
amino acids |
FLIPs for amino acids |
FRET-based biosensors | fluorescence microscopy, spectroscopy | cross-binding of other aa | Yeast cells | circularly permutated PBPs | eCFP, Venus | Ito T | |
| Metabolites |
inositol 1,4,5-trisphosphate |
IRIS-1 |
FRET-based biosensors | Tojyo Y | ||||||
| Metabolites |
PtdIns(3,5)P2 |
GFP-PHBtk |
Translocation-based biosensors | Balla T | ||||||
| Metabolites |
ATP |
ATeams |
FRET-based biosensors | spectroscopy, fluorescence microscopy | ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. | HeLa cells, NIH 3T3 | ? subunit from Bacillus subtilis FoF1-ATP synthase | CFP (mseCFP), YFP (cp173-mVenus) | Noji H |