Filters
Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs Addgene Link
Kinase Activity

Abl

EGFR indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific, sensitive to concentration of EGF B82, NIH 3T3, HeLa cells SH2 domain (residues 374–465) from mouse p52 Shc eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Src

Srcus

FRET-based biosensors HeLa cells, MEF cells CFP, YFP Chien S
Kinase Activity

Abl

Src indicator

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) NIH 3T3, HeLa Cells Rous sarcoma virus Src gene (residues148–245) eCFP, eYFP (citrine) Tsien RY
Kinase Activity

Abl

Picchu

FRET-based biosensors fluorescence microscopy Kinase Specific (tyrosine) HeLa cells, Cos7/E3 Src-homology (SH) 2 of Crk II or phosphotyrosine-binding (PTB) eYFP, CFP Matsuda M
Kinase Activity

Abl

Picchu-Z/EGFR-Z

FRET-based biosensors confocal flourescence microscopy, spectroscopy Kinase Specific (tyrosine) Cos1 and HT1080 and NIH3T3 and A431 Src-homology (SH) 2 and SH3 from Crk II YFP, CFP Matsuda M
Kinase Activity

ATM-Protein Kinase

ATOMIC

FRET-based biosensors confocal flourescence microscopy irreversible HeLa cells, NIH 3T3, AT4Bi Rad53 FHA2 (Chk2) and peptide with T68 phosphorylation site CFP, YFP Hunter T
Kinase Activity

Aurora B kinase

aurora B sensor

FRET-based biosensors confocal flourescence microscopy Xenopus S3, HeLa Cells, DU145, DLD21 reversible binding to an FHA2 phospho-threonine binding domain CFP, YFP Kapoor TM
Kinase Activity

CyclinB-Cdk1

CyclinB-Cdk1 sensor

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific HeLa cells, Xenopus, MEF, hTert-RPE1 cells Polo box domain (PBD) of PIk1 CFP(mCerulean) , YFP(YPet) Pines J
Mechanical Force

Mechanical strain

VinTS

FRET-based biosensors confocal microscopy BAECs, MEF cells, HEK293 cells mTFP1, venus(A206K) Schwartz MA
Mechanical Force

Mechanical strain

stFRET

FRET-based biosensors Dictyostelium , HS1, AX2 GFP, eGFP Uyeda TQ
Mechanical Force

Mechanical strain

PriSSM (PRIM-based)

FRET-based biosensors BAECs, HEK cells Cerulean, Venus Sachs F
Mechanical Force

Mechanical strain

sstFRET

FRET-based biosensors confocal microscopy HEK-293, 3T3, C.Elegans Cerulean, Venus Sachs F
Metabolites

1,2-diacylglycerol

DAGR (FRET)

Translocation-based biosensors Newton AC
Metabolites

histidine

FLIP-HisJ/cpHisJ194

FRET-based biosensors Ito T
Metabolites

PtdIns(3,5)P2

GFP-PHPDK1

Translocation-based biosensors Alessi DR
Metabolites

arabinose

FLIParaF.Ec-250n

FRET-based biosensors spectroscopy Kd-230nM ± 18 nM E. coli BL21-Gold(DE3) E.coli K12 araF eCFP, Venus Frommer WB Available
Metabolites

inositol 1,4,5-trisphosphate

LIBRA

FRET-based biosensors Mikoshiba K
Metabolites

PtdIns(3,5)P2

YFP-PHTAPP1

Translocation-based biosensors Alessi DR
Metabolites

arginine

FRET arginine reporter

FRET-based biosensors confocal flourescence microscopy, spectroscopy pH-sensitive, ornithine Kd-9.2 mM, Arg Kd-3.2 mM E. coli BL21(DE3)Gold, Arabidopsis thalina Protoplasts and roots bacterial glutamine binding protein (QBP) from E. coli with T70A and D157N eCFP, Citrine (shortened) Ludewig U
Metabolites

inositol 1,4,5-trisphosphate

FIRE1-3

FRET-based biosensors Mignery GA
Metabolites

PtdIns(3,5)P2

GFP-Svp1p

Translocation-based biosensors Lemmon MA
Metabolites

amino acids

FLIPs for amino acids

FRET-based biosensors fluorescence microscopy, spectroscopy cross-binding of other aa Yeast cells circularly permutated PBPs eCFP, Venus Ito T
Metabolites

inositol 1,4,5-trisphosphate

IRIS-1

FRET-based biosensors Tojyo Y
Metabolites

PtdIns(3,5)P2

GFP-PHBtk

Translocation-based biosensors Balla T
Metabolites

ATP

ATeams

FRET-based biosensors spectroscopy, fluorescence microscopy ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. HeLa cells, NIH 3T3 ? subunit from Bacillus subtilis FoF1-ATP synthase CFP (mseCFP), YFP (cp173-mVenus) Noji H

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