Sensor Target Type |
Specificity | Sensor Name | Sensor Type | Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs |
|---|---|---|---|---|---|---|---|---|---|
| Metabolites |
PtdIns(3,5)P2 |
GFP-Ent3p |
Translocation-based biosensors | Letourneur F | |||||
| Metabolites |
ATP |
ATeams |
FRET-based biosensors | spectroscopy, fluorescence microscopy | ATeams Kd - 7.4 µM and 1.7 (AT3.10), 14 µM and 2.0 (AT3.10MGK), 1.2 mM and 2.1 (AT1.03YEMK), and 3.3 mM and 2.1 (AT1.03), respectively. | HeLa cells, NIH 3T3 | ? subunit from Bacillus subtilis FoF1-ATP synthase | CFP (mseCFP), YFP (cp173-mVenus) | Noji H |
| Metabolites |
maltose |
FLIPmal sensors |
FRET-based biosensors | confocal flourescence microscopy | arabinose FLIP Kd-200 nM,maltose FLIP Kd-40 µM | Escherichia coli BL21-Gold(DE3) cells | periplasmic binding protein for arabinose or maltose, E. coli high-affinity L-arabinose binding protein AraF | eCFP, eYFP (venus) | Frommer WB |
| Metabolites |
PtdIns(4,5)P2 |
GFP-ARNO |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
ATP:ADP ratio |
Perceval |
Single FP-based extrinsic biosensors | Yellen G | |||||
| Metabolites |
NO |
FRET-MT |
FRET-based biosensors | Levitan ES | |||||
| Metabolites |
PtdIns(4,5)P2 |
GFP-Cytohesin-1 |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
BAI2 (a furanosyl borate diester) |
CLPY |
FRET-based biosensors | fluorescence microscopy | 98 kDa (CLPY), BAI2 Kd 270 nM | BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs | LuxP-BAI2 binding | CFP, YFP | Sayre R |
| Metabolites |
Phosphatidic acid |
Pii |
FRET-based biosensors | Kiyokawa E | |||||
| Metabolites |
PtdIns(4,5)P2 |
GFP-PHOSH2 |
Translocation-based biosensors | confocal microscopy | HeLa cells, yeast cells | eGFP | Lemmon MA | ||
| Metabolites |
BAI2 (a furanosyl borate diester) |
LuxP-FRET |
FRET-based biosensors | spectroscopy, fluorescence microscopy | BAI2 Kd 270 nM | Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) | LuxP-BAI2 binding | CFP, YFP | Sayre R |
| Metabolites |
Phosphatidylserine |
GFP-Lact-C2 |
Translocation-based biosensors | Grinstein S | |||||
| Metabolites |
PtdIns(4,5)P2 |
PHOSBP |
Translocation-based biosensors | confocal microscopy | COS-7 cells | GFP, mRFP | Balla T, Munro S | ||
| Metabolites |
cAMP |
FP-PKARII/FP-PKAcat |
FRET-based biosensors | eBFP and GFP or CFP and YFP | Pozzan T, Pozzan T | ||||
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-ARNO |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
PtdIns(4,5)P2 |
GFP-PHFAPP |
Translocation-based biosensors | confocal microscopy | HeLa cells, yeast cells | eGFP | Lemmon MA | ||
| Metabolites |
cAMP |
CFP–Epac–YFP |
FRET-based biosensors | fluorescence microscopy | highly sensitive cAMP indicator, Kd of 2.3 µM | human A431 cells, Rat-1 and NIH3T3 fibroblasts, mouse GE11 epithelial cells, mouse N1E-115 neuroblastoma, human MCF7 breast carcinoma cells | cAMP binding | CFP, YFP | Jalink K |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHGRP1 |
Translocation-based biosensors | confocal microscopy | PC12 cells | GFP | Cullen PJ, Czech MP | ||
| Metabolites |
PtdIns(4,5)P2 |
CAY |
FRET-based biosensors | Swiss 3T3 fibroblasts | Allen PG | ||||
| Metabolites |
cAMP |
ICUEs |
FRET-based biosensors | eCFP, Citrine | Zhang J, Zhang J, Lefkowitz RJ | ||||
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-Cytohesin-1 |
Translocation-based biosensors | Czech MP | |||||
| Metabolites |
cAMP |
Epac-camps |
FRET-based biosensors | spectroscopy, fluorescence microscopy | sensitive cAMP indicators | Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells | cAMP binding | eCFP, eYFP | Lohse MJ |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHPDK1 |
Translocation-based biosensors | Alessi DR, van Aalten DM | |||||
| Metabolites |
PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics |
GFP-PHPLC- |
Translocation-based biosensors | Nash MS | |||||
| Metabolites |
cAMP |
PKA-camps |
FRET-based biosensors | spectroscopy, fluorescence microscopy | sensitive cAMP indicators | Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells | cAMP binding | eCFP, eYFP | Lohse MJ |