Sensor Target Type |
Specificity | Sensor Name | Sensor Type | Analysis Technique | IV Properties | Organism | Binding Protein | Fluorophore | PIs |
|---|---|---|---|---|---|---|---|---|---|
| Metabolites |
cAMP |
PKA-camps |
FRET-based biosensors | spectroscopy, fluorescence microscopy | sensitive cAMP indicators | Sf9 cells, CHO-A2B, TsA201, HEK293, COS-7 cells | cAMP binding | eCFP, eYFP | Lohse MJ |
| Metabolites |
PtdIns(3,4,5)P3 |
YFP-PHTAPP1 |
Translocation-based biosensors | Alessi DR | |||||
| Metabolites |
PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics |
CYPHR (FRET: PLC activity) |
Translocation-based biosensors | Shapiro L | |||||
| Metabolites |
cAMP |
HCN2-camps |
FRET-based biosensors | confocal flourescence microscopy, FRAP | cAMP-sensitive | HEK 293a cells, mouse heart | single cAMP binding of HCN2 channel- a helices covering cAMP binding site | eCFP, eYFP | Engelhardt S |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-Svp1p |
Translocation-based biosensors | Lemmon MA | |||||
| Metabolites |
PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics |
FP-Tubby |
Translocation-based biosensors | Meyer T | |||||
| Metabolites |
cAMP |
(T)Epac(VV) |
FRET-based biosensors | FLIM, confocal fluorescence microscopy | cAMP indicators | Hek293 cells, N1E-115, human HeLa cells (ccl-2) | cAMP binding | mTurquise (mECFPD-Epac(CD, and DEP)-cp173Venus-Venus | Jalink K |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHBtk |
Translocation-based biosensors | Balla T | |||||
| Metabolites |
PtdIns(4,5)P2:Ins(1,4,5) P3 dynamics |
FP-PHPLCδ1(FRET) |
Translocation-based biosensors | Meyer T | |||||
| Metabolites |
cGMP |
FlincG |
Single FP-based extrinsic biosensors | Dostmann WR | |||||
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-PHCRAC |
Translocation-based biosensors | Snyder SH | |||||
| Metabolites |
PtdIns(4)P |
Pippi-PI(4)P |
FRET-based biosensors | MDCK cells | Matsuda M | ||||
| Metabolites |
cGMP |
Cygnets |
FRET-based biosensors | confocal flourescence microscopy | cGMP Kd - 600 nM, cAMP Kd - 300 µM | rat aortic smooth muscle cells, Sf9 insect cells, RFL-6 fibroblast cells | PKG-Ia binding | eCFP, Citrine | Dostmann WR |
| Metabolites |
PtdIns(3,4,5)P3 |
GFP-Ent3p |
Translocation-based biosensors | Letourneur F | |||||
| Metabolites |
PtdIns3P |
GFP-FYVEEEA1 |
Translocation-based biosensors | Emr SD | |||||
| Metabolites |
cGMP |
CGYs |
FRET-based biosensors | confocal flourescence microscopy | CHO-K1 cells, HEK-293 cells | PKG-Ia binding | eCFP, eYFP | Umezawa Y | |
| Metabolites |
PtdIns(3,4,5)P3 |
CAY |
FRET-based biosensors | Swiss 3T3 fibroblasts | Allen PG | ||||
| Metabolites |
PtdIns3P |
GFP–p40phox iPX |
Stephens LR | ||||||
| Metabolites |
cGMP |
cGES sensors |
FRET-based biosensors | confocal flourescence microscopy | HEK-293 cells | GAF-B domain of PDE2A and GAF-A domain of PDE5A | eCFP, eYFP | Lohse MJ | |
| Metabolites |
PtdIns(3,4,5)P3 |
Pippi-PI(3,4,5)P3 |
FRET-based biosensors | MDCK cells | Matsuda M | ||||
| Metabolites |
PtdIns4P |
GFP-PHOSH2 |
Translocation-based biosensors | confocal microscopy | Levine TP | ||||
| Metabolites |
cGMP |
cGi sensors |
FRET-based biosensors | spectroscopy, fluorescence microscopy | cGMP concentration 1-2 µM | HEK293 cells | cGMP-binding sites cNMP-BD (cGKI) or GAF (PDE5) | eCFP, eYFP | Koesling D |
| Metabolites |
PtdIns(3,4,5)P3 |
Fllip |
FRET-based biosensors | CHO cells | Umezawa Y | ||||
| Metabolites |
PtdIns4P |
PHOSBP |
Translocation-based biosensors | confocal microscopy | COS-7 cells | GFP, mRFP | Balla T, Munro S | ||
| Metabolites |
cGMP |
Cygnus |
FRET-based biosensors | FLIM, confocal fluorescence microscopy, spectroscopy | pH-sensitive | PC12 cells, HEK 293T cells, HeLa cells | cGMP binding from PDE5 | mTagBFP, sREACh(dark) | Oka K |