Filters
Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

ribose

FLIPrib sensors

FRET-based biosensors Lalonde S
Metabolites

diacylglycerol

Daglas

FRET-based biosensors fluorescence microscopy, spectroscopy Kinase Specific MDCK cells, NIH 3T3 C1 domain of PKCßII as DAG binding domain CFP, YFP Matsuda M
Metabolites

PtdIns(3,4)P2

GFP-PHGRP1

Translocation-based biosensors confocal microscopy PC12 cells GFP Cullen PJ
Metabolites

sucrose

FLIPsuc sensors

FRET-based biosensors Frommer WB
Metabolites

diacylglycerol

DAGR

FRET-based biosensors confocal flourescence microscopy MDCK cells Cysteine-rich domain (CRD) CFP, YFP Umezawa Y
Metabolites

PtdIns(3,4)P2

GFP-PHPDK1

Translocation-based biosensors Alessi DR, van Aalten DM
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Protein Activation and Conformation

Akt conformation

ReAktion

FRET-based biosensors fluorescence microscopy NIH3T3 fibroblasts human Akt-1/protein kinase B-a eCFP, Citrine Zhang J
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y
Protein Activation and Conformation

ERK conformation

Miu2

FRET-based biosensors HeLa cells, COS-7 cells, 293F CFP, YFP, RFP(Dropna) Matsuda M
Protein Activation and Conformation

Estrogen receptor ligand binding domain conformation

CEY

FRET-based biosensors COS-7 cells, Sf9 cells, Hf7c cells ER-Ligand binding domain seCFP, eYFP Lannigan DA
Protein Activation and Conformation

G protein activation

FP-Gα/FP-Gβ(γ)

FRET-based biosensors HeLa cells, HEK cells, HEK293T, Dictyostelium discoideum cells, FRT cells, haploid yeast cells YFP(Gaq-mYFP) and CFP(mTq), YFP and CFP, eYFP Gadella TW Jr, Lohse MJ, Bünemann M, Gilman AG, Bünemann M, Devreotes P, Scarlata S, Simon MI
Protein Activation and Conformation

Insulin receptor activation

Sinphos

Single FP-based extrinsic biosensors CHO-IR cells, CHO-K1 cells CFP(cpFP), GFP(cpFP) Umezawa Y
Protein Activation and Conformation

M(1) muscarinic receptor (M(1)R) activation

M1R-YFP-CFP

FRET-based biosensors fluorescence microscopy tsA-201 cells eCFP, eYFP Hille B
Protein Activation and Conformation

MAP kinase-activated protein kinase 2 conformation

GMB

FRET-based biosensors HEK293 cells, Swiss 3T3 cells eCFP, eBFP Gaestel M
Protein Activation and Conformation

PKB conformation

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 eGFP , RFP Larijani B
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rac

FRET-based biosensors COS-1 cells, HEK293 cells, 293T cells, HT1080 cells, HeLa cells CFP and YFP Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Cdc42

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-CRIB

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RhoA

FRET-based biosensors Matsuda M

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