Filters
Sensor Target Typesort descending Specificity Sensor Name Sensor Type Analysis Technique IV Properties Organism Binding Protein Fluorophore PIs
Metabolites

PtdIns(4,5)P2

GFP-Cytohesin-1

Translocation-based biosensors Czech MP
Metabolites

BAI2 (a furanosyl borate diester)

CLPY

FRET-based biosensors fluorescence microscopy 98 kDa (CLPY), BAI2 Kd 270 nM BL21 (luxS-) cells transformed with pQE30-CLPY or LuxP-mutant constructs LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

NO

FRET-MT

FRET-based biosensors Levitan ES
Metabolites

PtdIns(4,5)P2

GFP-PHOSH2

Translocation-based biosensors confocal microscopy HeLa cells, yeast cells eGFP Lemmon MA
Metabolites

BAI2 (a furanosyl borate diester)

LuxP-FRET

FRET-based biosensors spectroscopy, fluorescence microscopy BAI2 Kd 270 nM Escherichia coli BL21 (luxS-), V. harVeyi strains BB120 (wild-type), MM30 (luxS-), MM32 (luxS-, luxN-) LuxP-BAI2 binding CFP, YFP Sayre R
Metabolites

Phosphatidic acid

Pii

FRET-based biosensors Kiyokawa E
Neurotrophic Factor

Neurotrophic factor

BBD-ECat/ECaus (cell-based)

FRET-based biosensors CHO-EGFR cells , MCF-7 cells CFP, YFP Umezawa Y
Protein Activation and Conformation

M(1) muscarinic receptor (M(1)R) activation

M1R-YFP-CFP

FRET-based biosensors fluorescence microscopy tsA-201 cells eCFP, eYFP Hille B
Protein Activation and Conformation

MAP kinase-activated protein kinase 2 conformation

GMB

FRET-based biosensors HEK293 cells, Swiss 3T3 cells eCFP, eBFP Gaestel M
Protein Activation and Conformation

PKB conformation

GFP-PKB-RFP

FRET-based biosensors NIH 3T3 eGFP , RFP Larijani B
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rap1

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Rac

FRET-based biosensors COS-1 cells, HEK293 cells, 293T cells, HT1080 cells, HeLa cells CFP and YFP Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-Cdc42

FRET-based biosensors Matsuda M, Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-CRIB

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RhoA

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RBD

FRET-based biosensors Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

Raichu-RalA

FRET-based biosensors COS-7 cells CFP, YFP Matsuda M
Protein Activation and Conformation

Rho family GTPase activation

RhoA biosensor commentary

FRET-based biosensors Hahn KM, Hahn KM
Protein Activation and Conformation

Wiskott -Aldrich syndrome protein (WASP) conformation

Cdc42-GEF sensors

FRET-based biosensors NIH 3T3 cells eCFP, eYFP Rosen MK
Protein Activation and Conformation

activation of plasma membrane Ca2+ pump

BFP-PMCA-GFP

FRET-based biosensors spectroscopy concentration of BFP-PMCA-GFP-26 nM, PMCA-GFP-30 nM Saccharomyces cerevisiae strain DBY 2062, yeast cells Ca2+-calmodulin binding BFP, GFP Adamo HP
Protein Activation and Conformation

Akt conformation

ReAktion

FRET-based biosensors fluorescence microscopy NIH3T3 fibroblasts human Akt-1/protein kinase B-a eCFP, Citrine Zhang J
Protein Activation and Conformation

Akt conformation

GFP–Akt–YFP

FRET-based biosensors FLIM NIH 3T3 protein kinase B (PKB)/Akt eGFP , eYFP Larijani B
Protein Activation and Conformation

Annexin A4 self-association

CYNEX4

FRET-based biosensors confocal flourescence microscopy, FRAP HeLa cells, N1E-115, MDCK2 neuroblastoma cells Annexin A4 (Ca2+ regulated phospholated) eCFP, eYFP Schultz C
Protein Activation and Conformation

CaMKII conformation

CamuiαamCYCaMIIα

FRET-based biosensors two-photon fluorescence microscopy HeLa cells YFP(Venus) , CFP (S175G) Schultz C, Hayashi Y

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